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plasmids pet 28a mscarlet  (Addgene inc)


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  • 90

    Structured Review

    Addgene inc plasmids pet 28a mscarlet
    Plasmids Pet 28a Mscarlet, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet+28a+mscarlet+plasmid/pET-28a(%2B)-AACT+(Plasmid+%23108944)/pm36123532-302-0-14
    Average 90 stars, based on 3 article reviews
    plasmids pet 28a mscarlet - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Oligomerization state of the functional bacterial twin-arginine translocation (Tat) receptor complex.
    Article Snippet: .. The pET-28a_mScarlet plasmid was created by amplifying mScarlet from pCytERM mScarlet N1 (Addgene #85066) with an N-terminal 6xHis-tag and an SpeI site between the 6xHis-tag and mScarlet and inserting the H6-SpeI-mScarlet PCR product into pET28a (Novagen #69864) using NcoI and BamHI. .. The mNeon coding sequence amplified from plasmid L40C-CRISPR.EFS.mNeon (Addgene #69146) was then used to replace mScarlet using SpeI and BamHI, generating plasmid pH6-mNeon.

    Polymerase Chain Reaction:

    Article Title: Oligomerization state of the functional bacterial twin-arginine translocation (Tat) receptor complex.
    Article Snippet: .. The pET-28a_mScarlet plasmid was created by amplifying mScarlet from pCytERM mScarlet N1 (Addgene #85066) with an N-terminal 6xHis-tag and an SpeI site between the 6xHis-tag and mScarlet and inserting the H6-SpeI-mScarlet PCR product into pET28a (Novagen #69864) using NcoI and BamHI. .. The mNeon coding sequence amplified from plasmid L40C-CRISPR.EFS.mNeon (Addgene #69146) was then used to replace mScarlet using SpeI and BamHI, generating plasmid pH6-mNeon.



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    Addgene inc plasmids pet 28a mscarlet
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